prokaryotic system meaning in Chinese
原核系统
Examples
- Because of its special function , no successful example evolving the expression of antibacterial peptide directly in the prokaryotic system havs bben reported until now
由于抗菌肽特殊的功能特点，所以至今还没有能够在原核系统胞内直接成功表达的范例。 - It has not only many advantages of the prokaryotic expression system such as growing quickly , manipulating easily , strong power to express and control foreign protein , but also the ability to make foreign protein modified in post - translation . so pichia pastoris gene expression system is more appropriate to express many eukoryotic proteins than prokaryotic system and has attracted more and more people " s attention on it
它不仅具有原核表达系统生长快速，操作简单，外源基因表达量大并受到严格调控等特点，还具有真核生物特有的蛋白质翻译后修饰的功能，表达真核生物蛋白质具有原核表达系统无法比拟的优越性。 - To prepare the wild type mbl in prokaryotic system , a pair of primers was designed and synthesized , and was used to amplify mbl gene from the recombinant vector pgem - mbl that contans wild type mbl cdna . a recombinant prokaryotic expression vector , pet28 - mbl , was constructed by inserted the mbl gene into plasmid pet28 ( b ) , and after transfected it into ecoli bl21 ( de3 ) and induced with iptg , recombinant mbl protein was expressed successfully
本实验另选用了原核表达质粒pet28 ( b ) ，根据已构建好的含有mbl野生型基因的t载体pgem - mbl ，设计一对引物， pcr扩增mbl基因，凝胶回收，双酶切pcr产物和pet28 ( b )质粒， t4连接酶连接，转化大肠杆菌dhsa ，氨芋选择培养挑取克隆鉴定。